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Foliage Sample Collection Guide
 

Quality Improvement Laboratory

FOILAGE SAMPLE COLLECTION GUIDE



General Tips
Plant tissue samples must be collected properly and carefully to be useful for evaluating the nutrient status of a crop. Analytical results can only be as accurate as the sample. The most critical objective of sample collection is ensuring that it is representative of the area in question. If a field, orchard or vineyard is not uniform regarding soil type, fertilization, spray or disease conditions, separate samples from each distinct area are recommended. Do not combine specimens from different varieties, cultivars, ages or plantings. Best sample-collection method depends on the type of plant or crop being submitted. Recommendations for some crops are listed on this page. Please contact our lab for sample collection recommendations for other crops.

Field crops and vegetables - Leaf samples
During the morning hours, select the newest but fully expanded leaves on the main stem. Typically, this will be the third to fifth leaf branch from the growing tip of the plant. Avoid atypical insect- or weather-damaged plants unless special samples are being collected to assess a specific problem. Place the leaves in a Foliage Sample bag. Collect approximately 10 leaves from each of 3 to 5 areas within the field to be sampled. A minimum of 60 to 100 leaves should be submitted for a complete nutrient panel. Place leaves in a labeled Foliage Sample bag and submit to the lab within 24 hours for best results. Sample on a 7 to 14-day interval.

Field crops and vegetables - Petiole samples
During the morning hours, select the petioles from the newest but fully expanded leaves on the main stem. Typically, this will be the third to fifth petiole from the growing tip of the plant. Avoid atypical insect- or weather-damaged plants unless special samples are being collected to assess a specific problem. Strip away the leaf blades from the petioles and discard them. Place the petioles in a labeled Foliage Sample bag. Collect approximately 20 petioles from each of 3 to 5 areas within the field to be sampled. A minimum of 60 petioles should be submitted for a nitrate analysis, 100 for a complete nutrient panel. Sample on a 7 to 14-day interval. Submit the samples to the lab within 24 hours for best results.

Tree and orchard samples
Sample trees between 60 and 70 days after the average petal fall date. Select 60 to 100 leaves with petioles from the middle of terminal shoots. Shoots should be of average vigor representing typical trees in a planting with uniform soil, fertilizer and spray conditions. Avoid atypical insect- or weather-damaged shoots unless special samples are being collected to assess a specific problem. Select 1 or 2 leaves per shoot from shoots that are well exposed to light and 5 to 7 feet above ground level for large trees, or 3 to 6 feet above ground level for smaller trees. Beyond 70 days after petal fall, select the first fully mature leaf from the shoot tip. Place leaves in a labeled Foliage Sample bag and submit to the lab within 24 hours for best results. Wash spray residue and dirt from leaves before wilt if necessary.

File written by Adobe Photoshop® 4.0Berries and small fruits
Strawberries - Sample first fully matured leaf blades in June from fruiting plants or in early August from non-fruiting plants. A minimum of 75 leaf blades is required. Raspberries - Sample leaves on non-fruiting canes during August. A minimum of 75 leaf blades is required. Blueberries - Sample from July through August. A minimum of 150 leaf blades is required.
Select healthy leaves that have been well exposed to light that represent the average condition of the planting unless special samples are being collected to assess a specific problem. Collect 1 leaf per plant. A simultaneous soil pH test is recommended. Place leaves in a labeled Foliage Sample bag and submit to the lab within 24 hours for best results.

Grapes
Sample fruit-bearing grapevines during July to early September or at bloom for nitrogen status. Select petioles only from mature leaves opposite bunches. Immature or senescent leaves should not be sampled. Remove the leaf blade from the petiole and discard. Place 100 to 200 petioles in a labeled Foliage Sample bag and submit to the lab within 24 hours.

Mint
During the morning hours, select petioles from the newest but fully expanded leaves. Avoid atypical insect- or weather-damaged plants unless special samples are being collected to assess a specific problem. Strip away the leaves from the petioles and place them in a labeled Foliage Sample bag. Collect approximately 20 petioles from each of 3 to 5 areas within the field to be sampled. A minimum of 60 petioles should be submitted for a nitrate analysis, 100 for a complete nutrient panel. Submit the samples to the lab within 24 hours for best results. Sample on a 7 to 14-day interval.


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